BIOCHEMISTRY: Thin Layer Chromatography of Bilirubin

Saturday, May 14, 2011

Thin Layer Chromatography of Bilirubin

Bilirubin is seperated by Thin Layer Chromatography, after Van den Berge's reaction. (Pink -Diglucuronide; Bule - Monoglucuronide)

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LDL-Cholesterol Calculator

LDL Cholesterol Calculator Click here for a calculator that uses mmol/L units.

LDL Cholesterol Calculator

Total Cholesterol (mg/dL)

HDL Cholesterol (mg/dL)

Triglycerides (mg/dL)

LDL = (mg/dL) as per Friedewald formula

LDL = (mg/dL) as per "Iranian" formula

Friedewald (1972) Formula: LDL = TC - HDL - TG/5.0 (mg/dL) Reference.

"Iranian" (2008) Formula: LDL = TC/1.19 + TG/1.9 – HDL/1.1 – 38 (mg/dL) Reference.

Comment:
For those of us on a low carbohydrate lifestyle - the "Iranian" formula used above can perhaps give a better "estimation" of LDL when our Triglycerides are nice and low. However the Iranian study was made with people with elevated cholesterol. Hopefully one day, a study may be done with "healthy" people (and thus develop a more accurate formula)!

TEST CASE: (to ensure calculator is working correctly on your computer).
Total Cholesterol = 201 (mg/dL)
HDL Cholesterol = 74 (mg/dL)
Triglycerides = 44 (mg/dL)
LDL (estimated) = 118.2 (mg/dL) as per Friedewald formula
LDL (estimated) = 86.79273048932494 (mg/dL) as per "Iranian" formula
Full accuracy is shown, normally we would round-off to 1 (mg/dL)

BIOCHEMISTRY

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Saturday, May 14, 2011

Thin Layer Chromatography of Bilirubin

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